Single-molecule side synthesis Side nanopore Sequencing (NSBS)

The basic principle of NSBS sequencing technology adopted today is to combine the protein nanopore detection method with the system of "synthesis and sequencing". Four different special modification chains are added to the base end of the nucleic acid chain to be tested. During the replication process of the nucleic acid chain to be tested by polymerase, four kinds of chains pass through the nanopore and produce different characteristic blocking currents. The characteristic blocking current is monitored in real time by IC-MEMS integrated chip, the current change information is collected, the types of replicated nucleotides are analyzed, and the sequence of nucleic acid to be tested is obtained.

1, low cost: the cost per Gbp (billion bases) can be as low as 5 yuan or less, reducing the cost of human whole genome sequencing to hundreds of yuan.

2. High data quality and high accuracy: the Single-pass accuracy of sequencing can reach 90%, without systematic errors (non-random errors), and the comparison accuracy of human genome can exceed 99.999% (Consensus accuracy), meeting clinical needs. Long read length: N50 read length can be up to tens of kbp, and it has a strong ability to span structural variation (SV), and the number of Reads follows Gaussian distribution with reading length, and the proportion of Good reads is high.

3. No GC bias: It can easily cross regions with abnormal GC concentration and has high genome coverage.

4, wide range of adaptation: can sequence DNA and RNA, Single-sequence accuracy (single-sequence accuracy) more than 99.9%; It can also directly complete base modification detection with single base resolution to meet the needs of epigenetics.

5, convenient and fast: simple database construction, high sequencing efficiency, fast data processing speed, can complete the whole process from sample preparation to report within a few hours; The chip can be reused, there is no need to collect samples for cost saving, and the flexibility is good.